Multi-Spectral Sorting Based on Visibly High-Risk Kernels Sourced from Another Country Reduces Fumonisin and Toxigenic Fusarium on Maize Kernels

Highlights Abstract Fusarium species infect maize crops leading to production of fumonisin by their toxigenic members. Elimination of microbes is critical in mitigating further post-harvest spoilage and toxin accumulation. The current study investigates the efficacy of a previously described multi-spectral sorting technique to analyze the reduction of fumonisin and toxigenic Fusarium species found contaminating maize kernels in Kenya. […]

Spectral kernel sorting based on high-risk visual features associated with mycotoxin contamination reduces aflatoxin and fumonisin contamination in maize from Ghana

Highlights Abstract Rapid single kernel analysis could enable physical sorting to remove mycotoxins from bulk grains. The purpose of this study was to use visual characteristics previously associated with aflatoxin and fumonisin contamination of maize kernels to calibrate a multi-spectral sorter and then sort mycotoxin contaminated lots. A total of 76 corn samples were collected from poultry farmers in the Dorma-Ahenkro area, Ghana. Paired […]

Efficacy of electron beam irradiation in reduction of mycotoxin-producing fungi, aflatoxin, and fumonisin, in naturally contaminated maize slurry

Highlights Abstract Maize is a staple food in Kenya. However, maize is prone to fungal infestation, which may result in production of harmful aflatoxins and fumonisins. Electron beam (eBeam) food processing is a proven post-harvest technology, but published literature is rare on the ability of eBeam to reduce mycotoxins in naturally contaminated maize samples. This study evaluated the efficacy of […]

Single kernel aflatoxin and fumonisin contamination distribution and spectral classification in commercial corn

Highlights Abstract Aflatoxin and fumonisin contamination distribution in corn is non-homogeneous. Therefore, bulk sample testing may not accurately represent the levels of contamination. Single kernel analysis could provide a solution to these problems and lead to remediation strategies such as sorting. Our study uses extensive single kernel aflatoxin (AF) and fumonisin (FM) measurements to (i) demonstrate skewness, calculate weighted sums […]

When to use one-dimensional, two-dimensional, and Shifted Transversal Design pooling in mycotoxin screening

Abstract While complex sample pooling strategies have been developed for large-scale experiments with robotic liquid handling, many medium-scale experiments like mycotoxin screening by Enzyme-Linked Immunosorbent Assay (ELISA) are still conducted manually in 48- and 96-well plates. At this scale, the opportunity to save on reagent costs is offset by the increased costs of labor, materials, […]

A Review of the Methodology of Analyzing Aflatoxin and Fumonisin in Single Corn Kernels and the Potential Impacts of These Methods on Food Security

Abstract Current detection methods for contamination of aflatoxin and fumonisin used in the corn industry are based on bulk level. However, literature demonstrates that contamination of these mycotoxins is highly skewed and bulk samples do not always represent accurately the overall contamination in a batch of corn. Single kernel analysis can provide an insightful level […]